Platelet-rich plasma is described as the concentration of platelet-rich plasma protein acquired from whole blood filtered to remove the other blood cells. The aim of injecting a patient with PRP is to promote the repair of tissues and help with bone growth. At the same time, it helps in solving other medical conditions like loss of hair, osteoarthritis ligament, and tendons damages repairs.
Various methods can be applied when it comes to the preparation of the PRP. But the focus here is on utilizing differential centrifugation to ensure that platelets can be isolated and concentrated relative to other plasma components. The right centrifugation formula is essential to optimizing platelets and the growth factor concentrations while maintaining the integrity and viability of the platelet. The following is the optimal protocol involved in the PRP preparation.
The Centrifugal Protocol For PRP Preparation Includes The Following
It is essential to understand the right considerations that should be made when coming up with the centrifuge model for concentrating platelets and growth factors. The swing-out centrifuge is the most effective in preparing the PRP. The reason for this choice is that it provides better room to separate the components of plasma according to their density. On top of that, it helps in lowering the cell trauma concentration and damage relative, which boosts the PRP quality.
For this optimal protocol, it would involve the use of centrifugation which would help in separating the red blood cells. After separating the red blood cells, the next step is the second centrifugation, which helps concentrate platelets suspended in the final plasma volume. There is a double centrifugation process of the PRP where whole blood is collected in tubes containing anticoagulants. In this phase, the procedure is performed at a constant acceleration to separate the red blood cells from the entire blood volume. After that first spin, the whole blood separates into three layers: the upper layer containing platelets and the whole blood cells, which become the center of the procedure to produce the PRP. The production of the PRP, the upper layer, and the superficially buffy coat, the thin layer in the three layers formed, are transferred to an empty sterile tube.
The second spin would include the collection of platelet pellets with a few red blood cells at the bottom of the tubes. Afterward, homogenized platelet pellets arrived through thorough mixing at the third volume of the plasma, which would discard the upper two-thirds of the sterile tube.
Although PRP has proven to be very important in helping with tissue and bone repairs, one of the challenges that clinicians face is standardizing the right or the optimal protocol that should be applied when dealing with PRP equipment and its preparation. The reason why most clinicians still face these challenges is due to the numerous methods of its separation. The discussion above provides the optimal and simple protocol to help clinicians with the PRP preparations. In the end, it will help with the improvement of the treatment quality.
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