Camelids are typically immunised with a combination of five to ten antigens, followed by sorting by panning antigen-specific binders to each component. In light of this, it should be able to immunise using a crude extract of any specific cell, which may contain a protein that is now unknown to be "active." The camelid will produce HCAbs in response to immunogenic proteins, and the cloned VHH library can then be panned on the same proteome extract to enrich for Nbs against any part of the crude extract. In order to find Nanobodies that bind to or regulate the 'activity' of a specific component of the mixture, individual clones can be screened by ELISA [19] or a functional assay after the last round of panning (see the section on "Nb-based phenotypic screening").
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https://bloggers-vision.blogspot.com/2023/02/nanobodies-are-designed-to-specifically.html
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