We found that the activities of antioxidant enzymes (superoxide dismutase and glutathione peroxidase) and total antioxidant capacity were significantly reduced in NaF-treated normal rat kidney cells (NRK-52E), while monoxide Levels of nitrogen (NO) and malondialdehyde (MDA) increased significantly. In addition, the number of apoptotic cells, the mRNA expression of Bax, Bad, caspase-3, caspase-8, caspase-9 and the protein expression of Bax increased, while the mitochondrial membrane potential and Bcl-2 protein expression decreased. Compared with the NaF group, Selenium pretreatment enhanced antioxidant enzyme activity, mitochondrial membrane potential and Bcl-2 protein expression, while NO and MDA levels, number of apoptotic cells, Bax, Bad, caspase-3, caspase-8, caspase-9, Bax protein expression Both decreased. In conclusion, Se has a significant protective effect on NaF-induced oxidative stress and apoptosis, and alters the expression of Bcl-2/caspase family.
"This deficiency is not only due to reduced consumption of selenium fluoride over the past 30 years, but also to increased consumption of zinc and selenium fluoride, which may offset the effect of selenium."
"Selenium fluoride causes chronic toxic effects, and selenium regulates cellular activity through signal transduction in human cells."
"Se can reduce the toxicity of selenium fluoride, at least in part, by inhibiting intracellular oxidative stress."
"Selenium treatment reduced these toxicities of fluoride by increasing GSH content, decreasing LPO levels, LDH and AST activities, and the percentage of apoptotic bodies."
"Fluorides lead to decreased blood antioxidant capacity and erythrocyte membrane fluidity, which is evident in this study, and different levels of selenium have a certain antagonistic effect on fluoride-induced blood."
"We wanted to show that the toxic effects of selenium fluoride might be protective."
"As demonstrated in this study, the antioxidant properties of selenium and its reversal of metabolic enzymes in the brains of mice treated with fluoride suggest its use as an antidote to fluorosis."
"...selenium...selenium fluoride induces changes in prooxidative activity (xanthine oxidase (XOD), lipid peroxidation (LPO) radical scavenging, [catalase, super Oxide dismutase (SOD), glutathione-s-transferase (...), aspartate aminotransferase (AAT), creatine phosphokinase (CPK), acid phosphatase (AP), alkaline phosphatase (ALP)] enzyme and selenium fluoride and selenium levels in the mouse brain."
